BIOLOGY 211 LA-4 Enzymes Case Study Essay Paper
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BIOLOGY 211 LA-4 Enzymes Case Study Essay Paper
BIOLOGY 211 LA-4 Enzymes Page 2 of 2
Effect of temperature on the activity of turnip peroxidase
(If you are struggling with any of the questions, I recommend thoroughly reading through the first two pages of Enzyme Lab introduction.)
Question:
Null hypothesis (avoid using the word “will” in your hypothesis): (If you need help with hypothesis or independent and dependent variables, I suggest reviewing background information from Diffusion Lab.)
In this experiment, what are the independent and dependent variables?
independent:
dependent:
What is the name of the enzyme in this enzyme-mediated reaction?
What is the substrate molecule in this enzyme-mediated reaction? _______________________
What is/are the product(s) produced in this enzyme-mediated reaction?
Which tube served as a negative control? __________________________
How do you know it is a negative control?
Paste in your graph below:
Do the data support the null hypothesis? Explain, using the data (numbers) you obtained to support your conclusion.
Of those tested, what is the optimum temperature for turnip peroxidase? _______________
Why is activity lower at other temperatures?
Effect of pH on the activity of turnip peroxidase
Question:
Null hypothesis (avoid using the word “will” in your hypothesis):
In this experiment, what are the independent and dependent variables?
independent:
dependent:
Which tube served as a negative control? __________________________
Which tube served as a positive control? (which would you to predict to have optimal pH for turnip peroxidase)
__________________________
Paste in your graph below:
Do the data support the null hypothesis? Explain, using the data (numbers) you obtained to substantiate your conclusion.
Of those tested, what is the optimum pH for turnip peroxidase? _______________
Why is activity lower at other pH values?
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